白介素-4对人气道壁血管平滑肌细胞 ADAM33基因表达的影响研究

摘要

Objective To study the effect of IL-4 on ADAM33 gene expression in airway wall vascular smooth muscle cells.Methods Human airway wall vascular smooth muscle cells were cultured and stimu-lated with 0.1,1.0,10 and 100 ng/mL IL-4.The expression of ADAM33 mRNA was evaluated by RT-PCR and its protein was evaluated by Western blotting.Results ADAM33 mRNA was expressed in airway wall vascular smooth muscle cells.IL-4 enhanced ADAM33 gene expression in airway wall vascular smooth muscle cells in a dose and time-dependent (IL-4 was at 100 ng/mL)manner.Conclusion It was suggested that ADAM33 gene was expressed on airway wall vascular smooth muscle cells and IL-4 could enhance ADAM33 expression.%目的：探讨白介素-4（IL-4）对人气道血管壁平滑肌细胞去整合素金属蛋白酶33（ADAM33）基因表达的影响。方法（1）以0．1、1．0、10、100 ng／mL IL-4刺激培养人气道壁血管平滑肌细胞，用实时定量反转录多聚酶链反应（RT-PCR）法及蛋白印迹法（Western Blot）测定 ADAM33基因表达的变化；（2）以100 ng／mL IL-4与人气道壁血管平滑肌细胞共培养，0、6、12、24、48、72 h 后，采用 RT-PCR 及 Western Blot 法测定 ADAM33 mRNA及蛋白表达的变化。结果人气道壁血管平滑肌细胞中存在 ADAM33 mRNA 的表达，当 IL-4刺激浓度增加时， ADAM33 mRNA 表达也增加，总体呈现出浓度依赖性趋势；当以100 ng／mL IL-4刺激时，随刺激时间延长，AD-AM33 mRNA 的表达也有增加，总体呈现时间依赖性趋势。结论 IL-4能促进人气道壁血管平滑肌细胞ADAM33基因的表达。