肉苁蓉苯乙醇总苷对Aβ1-42诱导的PC12细胞损伤的保护作用

摘要

目的 研究肉苁蓉苯乙醇总苷(PhGs)对Aβ1-42蛋白诱导致大鼠肾上腺嗜铬细胞瘤细胞(PC12)损伤的保护作用.方法 将培养的PC12细胞随机分为空白组、模型组、不同浓度的PhGs药物干预组(5、25、50μg/mL),药物干预细胞模型后,采用MTT比色法测定细胞存活率,酶标法测定乳酸脱氢酶(LDH)渗漏率.结果 Aβ1-42损伤模型最佳损伤条件为0.5μmol/L造模浓度损伤48h;建模过程中发现Aβ1-42蛋白诱导损伤细胞稳定高效,通过不同剂量肉苁蓉苯乙醇总苷干预,能明显改善Aβ1-42对PC12细胞模型造成的细胞损伤,与模型组比较,肉苁蓉苯乙醇总苷可提高PC12细胞存活率,减少LDH漏出量,差异有统计学意义(P<0.05).结论 肉苁蓉苯乙醇总苷对Aβ1-42诱导的PC12细胞损伤具有明显的保护作用.%Objective To study the protective effects of glycosides of cistanche (PhGs) on damage in PC12 cells induced by aggregated β-amyloid protein 1-42 (Aβ1-42).Methods PC12 cells were grouped into blank group, model group and drug intervention group (5, 25, 50 μg/mL) with different concentrations PhGs, after the intervention.the cell survival rates was examined by determined MTT colorimetric assay.And the lactate dehydrogenase (LDH) leakage rate was determined by enzyme labeled method.Results The optimum damage condition of the model is 0.5 μmol/L concentration of Aβ1-42 for 48 h.We found the modle is stable and efficient.While the phenylethanoid glycosides (5, 25, 50 μg/mL) can significantly ameliorate the cell injury caused by Aβ 1-42.Compared with the model group, PhGs was shown to be able to improve the cell survival rate and reduce the leakage of LDH.The difference was statistically significant (P<0.05).Conclusion Phenylethanoid glycosides was shown to have obvious neuroprotective effect on PC12 cells induced damage by β-amyloid protein 1-42.