门静脉高压症患者肝组织CD44、血清透明质酸和肝功能性血流量的变化

摘要

Objective To evaluate the function of hepatic sinusoidal endothelial cell by investigating changes of functional hepatic blood flow (FLPF) in patients with liver cirrhosis and portal hypertension (LCPH). Methods We recruited 50 LCPH patients and 10 liver cyst patients without cirrhosis who underwent operation in the same period. Immunohistochemical staining was employed to examine the expression of CD44. The content of serum hyaluronic acid was examined by radioimmunoassay and FLPF was determined by modified hepatic clearance of D-sorbitol. Results ①In control group the expression of CD44 decreased and the positive staining area was (3.49±0.38)μm2, while in LCPH group the expression of CD44 increased and the positive staining area was (41.38 ±11.88)μm2.②The content of serum hyalurouic acid was increased in LCPH group [(116.11 + 20.12)μg/L] as compared with that in control group [(48.15±14. 39)μg/L].③FLPF evaluated by means of D-sorbitol clearance was reduced in LCPH group [(770.33±48.53)mL/min] compared to that in control group [(1 261.22±65.79)mL/ min]. Conclusion The decrease of FLPF in LCPH patients is closely related to the change of the function of hepatic sinusoid endothelial cell.%目的 探讨乙肝肝硬化门静脉高压症( LCPH)患者肝窦内皮细胞功能与肝功能性血流量(FLPF)变化的关系.方法 选择50例LCPH患者和10例同期手术的非肝硬化肝囊肿患者,应用免疫组化法检测肝组织CD44的表达水平,放免法检测血清透明质酸的含量,D-山梨醇清除率法检测FLPF.结果 对照组肝组织CD44表达水平很低,阳性染色面积为(3.49±0.38)μm2,LCPH组则明显上调,达(41.38±11.88)μm2.对照组血清透明质酸含量为(48.15±14.39)μ g/L,LCPH组升高,达(116.11±20.12)μg/L.对照组FLPF为(1 261.22±65.79)mL/min,LCPH组降低至(770.33±48.53)mL/min.结论 LCPH患者肝窦内皮细胞功能的变化与FLPF的降低密切相关.