BIOTRANSPLANTAT, METHOD FOR ITS PRODUCTION, METHOD FOR TREATING chronic diffuse liver diseases and treatments tsiroz liver and portal hypertension

摘要

1.u0431u0438u043eu0442u0440u0430u043du0441u043fu043bu0430u043du0442u0430u0442 u0434u043bu00a0 u043bu0435u0447u0435u043du0438u00a0 chronic diffuse diseases of the liver in the form of suspension in physiological solution with the concentration of the cellular elements 2 to 4 million claix a current of 1 kg of the weight of the patient, u0445u0430u0440u0430u043au0442u0435u0440u0438u0437u0443u044eu0449u0438u0439u0441u00a0 that contains mixed u043fu043eu043fu0443u043bu00a0u0446u0438u044e cells obtained from liver tissue u0444u0435u0442u0430u043bu044cu043du043eu0439 9 - 11 and 16 to 22 weeks u0433u0435u0441u0442u0430u0446u0438u0438 with u0438u0441u043fu043eu043bu044cu0437u043eu0432 u0430u043du0438u0435u043c u0444u0435u0440u043cu0435u043du0442u0430u0442u0438u0432u043du043eu0433u043e u0440u0430u0437u0434u0435u043bu0435u043du0438u00a0 cells among needlean u0441u04302 + in physiological concentrations u043au043eu043bu043bu0430u0433u0435u043du0430u0437u0443 and u0434u043du043au0430u0437u0443, with 40 mas.% in the mixed u043fu043eu043fu0443u043bu00a0u0446u0438u0438 cells u0441u043eu0441u0442u0430u0432u043bu00a0u044eu0442 stem cells u0432u043au043bu044eu0447u0430u00a0 u0441u0442u0440u043eu043cu0430u043bu044cu043du044b (e u043cu0435u0437u0435u043du0445u0438u043cu0430u043bu044cu043du044bu0435 stem cells, hepatic stem cells u0431u0438u043fu043eu0442u0435u043du0442u043du044bu0435 itself, and the rest to 60 mas.percent of cells u0441u043eu0441u0442u0430u0432u043bu00a0u044eu0442 progenitor cell in different stages of differentiation, the cells u043fu0440u0435u0434u0441u0442u0430u0432u043bu00a0u044eu0449u0438u0435's predecessors, including the young immature hepatic u0433u0435u043fu0430u0442u043eu0431u043bu0430u0441u0442u044b cells and cells - before u044du0440u0438u0442u0440u043eu0438u0434u043du043eu0433u043e u0440u00a0u0434u0430 - u0433u0435u043cu043eu043fu043eu044du0442u0438u0447u0435u0441u043au0438u0435 cells.;2. method u043fu043eu043bu0443u0447u0435u043du0438u00a0 u0431u0438u043eu0442u0440u0430u043du0441u043fu043bu0430u043du0442u0430u0442u0430 on p.1, u0445u0430u0440u0430u043au0442u0435u0440u0438u0437u0443u044eu0449u0438u0439u0441u00a0, tissue u0444u0435u0442u0430u043bu044cu043du043eu0439 liver washed free from u0441u04302 + and mg2 + in the u0445u0435u043du043au0441u0430 with 0.05 m u044du0434u0442u0430 (u044du0442u0438u043bu0435u043du0434u0438u0430u043cu0438u043du043eu0442u0435u0442u0440u0430u0443u043au0441u0443u0441u043du0430u00a0 acid), u0438u043du043au0443u0431u0438u0440u0443u044eu0442 in some 37u0441 for 10 min in the same solution, u043eu0441u0443u0449u0435u0441u0442u0432u043bu00a0u044eu0442 u0444u0435u0440u043cu0435u043du0442u0430u0442u0438u0432u043du043eu0435 division of cells in the environment of processing needle containing u0441u04302 + in the physiological u043au043eu043du0446u0435u043du0442u0440u0430u0446 so, u043au043eu043bu043bu0430u0433u0435u043du0430u0437u0443 0.5 mg in the liver, 1 mg / ml u0434u043du043au0430u0437u044b,filtered through filter with the size of the pores to 90 microns, u0446u0435u043du0442u0440u0438u0444u0443u0433u0438u0440u0443u044eu0442 at 1000 rpm, derived from sediment and environmental u043fu043eu0432u0442u043eu0440u00a0u044eu0442 needle and the fermentation processing times with half the number of u0434u043du043au0430u0437u044b.;3. method u043bu0435u0447u0435u043du0438u00a0 chronic diffuse diseases of the liver, u0445u0430u0440u0430u043au0442u0435u0440u0438u0437u0443u044eu0449u0438u0439u0441u00a0, use u0431u0438u043eu0442u0440u0430u043du0441u043fu043bu0430u043du0442u0430u0442 on p.1, the way for p.2 containing a mixed u043fu043eu043fu0443u043bu00a0u0446u0438u044e cells comprising u0441u0442u0440u043eu043cu0430u043bu044cu043du044bu0435 u043cu0435u0437u0435u043du0445u0438u043cu0430u043bu044cu043du044bu0435 stem cells in hepatic u0431u0438u043fu043eu0442u0435u043du0442u043du044bu0435 stem cells, as well as ol u043eu0433u0435u043du0438u0442u043eu0440u043du044bu0435 cells at different stages of differentiation u0432u0432u043eu0434u00a0u0442 in the form of suspension in physiological solution with the concentration of the cellular elements 2 to 4 million cells per 1 kg the weight of the patient.;4. method u043bu0435u0447u0435u043du0438u00a0 cirrhosis of the liver and u043fu043eu0440u0442u0430u043bu044cu043du043eu0439 hypertension, u0445u0430u0440u0430u043au0442u0435u0440u0438u0437u0443u044eu0449u0438u0439u0441u00a0, use u0431u0438u043eu0442u0440u0430u043du0441u043fu043bu0430u043du0442u0430u0442 on 1, 2, and u0437u0430u043au043bu044eu0447u0430u044eu0449u0438u0439u0441u00a0 in the way the introduction of the transplant recipient, from 350 to 500 million u0431u0438u043eu0442u0440u0430u043du0441u043fu043bu0430u043du0442u0430u0442u0430 hybrid u043fu043eu043fu0443u043bu00a0u0446u0438u0438 u0444u0435u0442u0430u043bu044cu043du044bu0445 cells in the form of suspension in physiological solution.