白血病HL60/ADR细胞Mdrl、NF-κB的表达及解毒化瘀药的干预作用

摘要

Objective To discuss the influence of Jiedu Huayu Chinese herbal medicine on the expressions of multidrug resistance gene 1 (Mdrl) and nuclear factor kappa B (NF-κB) of leukemia HL60/ADR cell. Methods Semi-quantitative reverse transcriptase polymerase chain reaction method was used to examine the expression of Mdrl gene after HL60/ADR cell receiving the treatment of Jiedu Huayu Chinese herbal medicine, and Western blotting technology was applied to examine the expressions of NF-κB/P65, NF-κB/P50 and IκBα. Results The expressions of NF-κB/P65, NF-κB/P50 and IκBα and the expression of Mdrlof leukemia HL60/ADR cell could be decreased by Jiedu Huayu Chinese herbal medicine. Conclusion Jiedu Huayu Chinese herbal serum can reverse multidrug resistance in leukemia HL60/ADR cell by blocking NF-κB signal path and affecting the expression of Mdrl.%目的 探讨解毒化瘀药血清对白血病HL60/ADR细胞多药耐药基因(Mdrl)、核周因子κB(NF-κB)信号基因表达的影响.方法 采用半定量逆转录聚合酶链反应(RT PCR)法检测解毒化瘀药血清处理后HL60/ADR细胞Mdrl基因的表达；Western blot法检测NF-κB/P65、NFκB/P50、IκBα的表达.结果 解毒化瘀药血清能够降低HL60/ADR耐药细胞NFκB/P65、NFκB/P50、1κBα的表达,降低Mdrl耐药基因的表达.结论解毒化瘀药对HL60/ADR细胞的耐药逆转作用可能是通过阻断NF-κB信号通路,影响Mdrl耐药基因的表达,进一步逆转耐药.