目的 应用miRNA芯片技术筛选三阴性乳腺癌及癌旁组织差异表达的miRNAs,为进一步阐明其在三阴性乳腺癌发病机制中的作用奠定基础.方法 收集8例新鲜的三阴性乳腺癌及其癌旁组织,抽提总RNA进行Hy3荧光标记,miRCURYTM LNA Array (v.16.0) (Exiqon)芯片上进行杂交反应,实时定量PCR验证miRNAs芯片结果的可靠性.结果 对芯片进行图像扫描和数据分析,筛选获得17个三阴性乳腺癌相关miRNAs,其中7个miRNAs表达上调,10个miRNAs表达下调;显著上调的有miR-93、miR-328,显著下调的有miR-145、miR-205.结论 筛选得到三阴性乳腺癌miRNA差异表达谱,其可能与三阴性乳腺癌的发生发展有关.%Objective To screen and identify the miRNA differential expression profile in triple-negative breast cancer by the miRNA array technique. Methods Total RNA was extracted from 8 fresh breast tumor samples and normal adjacent tissues. The miRCURYTM LNA Array (v. 16.0) (Exiqon) was used to screen the differentially expressed miRNA. Real-time quantitative PCR was applied to verify the reliability of miRNA array results. Results Compared to normal adjacent tissues, 17 miRNAs were differentially expressed in triple-negative breast cancer tissues, including 7 up-regulated and 10 down-regulated miRNAs. The most significantly up-regulated miRNAs were miR-93 and miR-328, while miR-145 and miR-205 were significantly down-regulated. Conclusion We obtained miRNA differential expression profile of triple-negative breast cancer. The profile may be related to the pathogenesis and development of triple-negative breast cancer.
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