Objective To investigate the effects as well as the interaction between endothelial progenitor cells (EPCs) and vascular endothelial growth factor (VEGF) on the occurrence and development of proliferative diabetic retinopathy (PDR) by detecting the fibrovascular membranes in PDR patients. Methods We collected 10 PDR membranes stripped from vitreous surgery; 10 PVR membranes and 1 human retina served as controls. Morphology of the membranes was observed. VEGF expression and EPCs counting were observed by immunohistochemistry. Results PDR membranes consisted of the central avascular area, the middle fiber structure with a few cells, and the peripheral rich cell area. Cells were distributed disorderly in PVR membranes. EPCs were found in all the 10 PDR membranes (5.90±1.83) and in only 1 PVR membrane (0. 10±0. 32), but not in human retina. VEGF expression in PDR membranes was significantly higher than in the control group (P<0.001). The number of EPCs was positively correlated with VEGF expression in PDR membranes. Conclusion EPCs and VEGF participate in and have a synergistic effect on neovascularization in PDR.%目的 检测增生性糖尿病视网膜病变(PDR)患者纤维血管膜标本中血管内皮祖细胞和血管内皮生长因子(VEGF)的表达,探讨二者与PDR发生发展的关系及相互作用.方法 收集10例玻璃体手术中剥除的PDR膜,以10例增生性玻璃体视网膜病变(PVR)膜及1例人视网膜为对照,进行组织形态学观察,免疫组织化学染色检测血管内皮祖细胞数及VEGF的表达,并进行统计学分析.结果 PDR膜包括中央部无血管区,间有少量细胞的纤维组织区和周边部富细胞区,PVR膜则为排列不规则的细胞散在分布于细胞外间质.10例PDR膜中均发现血管内皮祖细胞(5.90±1.83)个,而10例PVR膜中仅有1例发现血管内皮祖细胞(0.10±0.32)个,人视网膜未见血管内皮祖细胞.PDR膜VEGF表达明显高于对照组,差异有统计学意义(P＜0.001),且PDR膜中血管内皮祖细胞数与VEGF的表达呈现正相关性.结论 血管内皮祖细胞与VEGF均参与了PDR新生血管的形成,二者可能具有协同作用.
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