MCM5和 P16INK4A在宫颈鳞癌组织中的表达及其临床意义

摘要

Objective To investigate the expressions of minichromosome maintenance protein 5 (MCM5)and P1 6 INK4A gene and protein in cervical squamous cell cancer (SCC),cervical intraepithelial neoplasia (CIN)and normal tissues.Methods Real-time PCR and immunohistochemistry were used respectively to detect the expression levels of mRNA and protein of MCM5 and P1 6 INK4A in 40 cases of cervical cancer,1 1 cases of CIN I,1 5 cases of CIN Ⅱ - Ⅲ and 1 5 cases of normal cervical tissues.Results ① The expression levels of mRNA and protein of MCM5 and P1 6 INK4A in tissues of cervical squamous cell cancer were significantly higher than those in normal cervical tissues,CIN I and CIN Ⅱ-Ⅲ tissues (P <0.05).The expression level of P1 6 INK4A was significantly higher in tissues of cervical squamous cell cancer than in normal cervical tissues and CIN I tissues (P < 0.01 ). However,it did not differ from that in CIN Ⅱ-Ⅲ tissues (P >0.05 ).② The high expression of MCM5 mRNA and protein in cervical squamous cell carcinoma was positively correlated with clinical stage and differentiation grade of cervical cancer (P <0.01),but not correlated with age (P >0.05).P1 6 INK4A expression exhibited no correlation with clinical stage or age of patients (P >0.05),but positive correlation with differentiation grade (P <0.01).③ A positive correlation was found between MCM5 and P1 6 INK4A expressions in cervical cancer (r = 0.538,P <0.01).Conclusion The over-expressions of MCM5 and P1 6 INK4A may play an important role in the carcinogenesis and progression of cervical cancer.MCM5 may be used as a new marker of the proliferation of cervical cancer. Detection of P1 6 INK4A and MCM5 is of great significance to studying the grade and outcome of CIN,which may improve the rate of cervical cancer screening.%目的：探讨微小染色体维持蛋白-5(MCM5)与 P16 INK4A 在宫颈癌组织、宫颈上皮内瘤变和正常宫颈组织中的表达及其意义。方法采用实时荧光定量 PCR(Real-time PCR)和免疫组化法,分别检测 MCM5和 P16 INK4A mRNA 及蛋白在40例宫颈癌组织、11例低度宫颈上皮内瘤变(CINⅠ)、15例高度宫颈上皮内瘤变(CINⅡ~Ⅲ)和15例正常宫颈组织中的表达。结果①各不同宫颈组织中 MCM5和 P16 INK4A mRNA 及蛋白的表达趋势一致。宫颈癌组织中MCM5和 P16 INK4A mRNA 的表达量和蛋白表达率均高于 CINⅠ、CINⅡ~Ⅲ和正常宫颈组织。MCM5基因和蛋白在宫颈癌组织中的表达均比正常宫颈组织、CINⅠ、CINⅡ~Ⅲ高,差异均具有统计学意义(P <0.05)；P16 INK4A 基因和蛋白在宫颈癌中的表达比正常宫颈组织、CINⅠ高,差异均有统计学意义(P <0.01),而与 CINⅡ~Ⅲ间差异无统计学意义(P >0.05)。②MCM5 mRNA 表达量和蛋白表达率与临床期别、分化程度相关,差异有统计学意义(P <0.01),与年龄无关(P >0.05)；P16 INK4A mRNA 和蛋白表达与临床期别、年龄无关(P >0.05),而与分化程度相关,差异有统计学意义(P <0.01)。③在宫颈癌组织中 MCM5和 P16 INK4A 表达呈正相关(r =0.538,P <0.01)。结论 MCM5和P16 INK4A 的高表达可能与宫颈癌的发生发展有关。MCM5可能成为宫颈癌肿瘤增生的新标志物；其与 P16 INK4A 联合检测有助于进行 CIN 的分级和转归判断,有望提高宫颈癌的筛查率。