过敏性鼻炎合并哮喘患者血液嗜酸性粒细胞群中P物质及其受体表达上调

摘要

Objective To detect the expression of substance P(SP)and NK1 receptor(NK1R)in eosinophils of patients with allergic rhinitis complicated with asthma(AR+ AS)and elucidate their roles in the pathogenesis. Methods Levels of SP and NK1R in eosinophils were detected by flow cytometry after stimulation with crude extracts of Artemisia pollen,dust mite and Platanus pollen,respectively.Results The proportion of SP+cells in patients with AR+AS was 1.5 folds higher than that of healthy controls(HCs)(Z= -2.041,P= 0.041).The ratio of NK1R+cells and the mean fluorescent intensity were increased by 26.4%(Z= -3.207,P=0.001)and 85.9%(Z= -4.774,P＜ 0.001),respectively.In addition,0.1 μg/mL of Artemisia pollen extract induced an increase of SP+eosinophils in AR+AS patients by approximately 68.1%(Z= -2.637,P=0.008).However,no significant difference was detected in the expressions of SP and NK 1R in blood eosinophils of HCs when stimulated with allergens.Conclusion Eosinophil-derived SP and NK1R may play an important role in the development of AR+AS.SP and NK1R may be the potential targets for AR+AS treatment.%目的 检测不同过敏原刺激下过敏性鼻炎合并哮喘(allergic rhinitis complicated with asthma,AR+AS)患者嗜酸性粒细胞群中P物质(substance P,SP)和NK1受体(neurokinin 1 receptor,NK1R)的表达水平,并阐述其在疾病发生过程中的作用.方法 募集14例健康人及19例急性期入院的AR+AS患者,所有志愿者进行皮肤点刺试验并收集外周血,用蒿草花粉、尘螨和梧桐花粉3种过敏原粗提液刺激后流式细胞术检测外周血嗜酸性粒细胞群中SP和NK1R的表达水平,并用SPSS软件进行数据分析.结果 19例AR+AS患者外周血嗜酸性粒细胞中,SP+细胞的比例与正常人相比升高了1.5倍(Z= -2.041,P=0.041),NK1R+细胞的比例和平均荧光强度分别上调了26.4%(Z=-3.207,P=0.001)和85.9%(Z= -4.774,P＜0.001).此外,质量浓度为0.1 μg/mL的蒿草花粉粗提液刺激患者外周血后,SP+细胞的比例上调约68.1%(Z= -2.637,P=0.008).而健康人嗜酸性粒细胞群中SP和NK1R经不同种类和浓度过敏原刺激后其表达水平无统计学差异.结论 嗜酸性粒细胞源SP和NK1R可能在AR+ AS的发生过程中起重要作用,而SP和NK1R可能是治疗AR+AS的新靶点.