目的 检测Sox11基因在宫颈癌细胞系和不同宫颈病变组织中的表达情况.方法 应用免疫组织化学法和Western blot技术检测Sox11蛋白在宫颈癌细胞系(HeLa、CaSki、SiHa和C-33A)以及在正常宫颈、低度及高度宫颈鳞状上皮内病变、宫颈浸润癌组织中的表达情况及差异,分析宫颈癌组织中Sox11蛋白表达与临床病理参数之间的相关性.结果 Sox11蛋白在正常宫颈、低度宫颈鳞状上皮内病变中的表达显著高于高度宫颈鳞状上皮内病变及宫颈浸润癌组织中的表达,其表达随宫颈病变的进展而降低;Sox11蛋白在宫颈浸润癌中的表达随恶性程度增加而降低;Sox11蛋白在宫颈浸润癌中的表达与HPV感染有关,与年龄、临床分期、淋巴结转移及浸润肌层深度无相关性.结论 Sox11表达与宫颈癌发生发展呈负相关,提示Sox11可能作为一个肿瘤抑制基因发挥作用,其表达缺失或低表达与宫颈癌的发生发展有关,是宫颈癌发生的早期事件,可能是宫颈组织恶变的一个信号.%Objective To explore the expression level of Sox11 gene in cervical cancer cell lines and cervical pathology tissue .Methods The expression of Sox11 protein was detected in cervical cancer cell lines HeLa , CaSki ,SiHa and C-33A as well as NC ,LSIL ,HSIL and CC using immunohistochemistry and Western blot .We analyzed the relationship between Sox11 expression in cervical cancer and clinicopathologic parameters .Results The expression of Sox11 protein in normal cervical tissue ,low-grade cervical squamous intraepithelial neoplasia was significantly higher than that in the high-grade cervical squamous intraepithelial neoplasia and invasive cervical cancer tissue , and the expression was reduced with the progression of cervical lesions .The expression of Sox11 protein in the invasive cervical cancer was reduced with increased malignant degree .The expression of Sox11 protein in the invasive cervical cancer was significantly correlated with HPV infection ,but not associated with the age , clinical stage ,lymph node metastasis or muscular infiltration depth .Conclusion Sox11 expression is negatively correlated with cervical cancer development ,suggesting that as a tumor suppressor gene ,Sox11 may play a role in cervical cancer development and its absence or low expression is associated with the development of cervical cancer and is an early event of cervical carcinogenesis and may be a sign of malignant change of cervical tissue .
展开▼
