To research the effect of cholesterol content on the nonalcoholic fatty liver disease ( NAFLD) induced by high fat.Method:The adult male SD rats were randomly divided into the normalgroup ( CON) and high-fat diet group (HF).Then the HF group was divided into HFC0, HFC1, HFC2 and HFC5 group, and 0%, 1%, 2%and 1%cholesterol were added into the five HFC groups correspondingly.All rats were beheaded at the 4th and 8th week, and the organ blood was collected to detect the contentsof the totalcholesterol (TC), the total triglyceride (TG) and the activities of Alanineaminotransferase (AST), Alanine transaminase(ALT).The 8th weeks’ liver tis-sues embedded inparaffin were stained with hematoxylin and eosin ( H&E) to judge inflammatorysituation.Result:After 8 weeks’ feeding,compared with the CON group, the body weight, liver weight and the proportion of liver and body weight of the HFC group rats increased significantly with the increase of cholesterol intake .And the liver mor-phology changes, lipid accumulation and inflammatory infiltration could be easily observed on HFD liver cells.At the 4th week, the content of TC, activities of ALT and AST increased significantly in the HFC2 and HFC5 group. Conclusion:The results showed the high cholesterol diet can aggravate hepatic lipid accumulation, liver dysfunctio-nand promote the development of nonalcoholic fatty liver disease.%研究胆固醇含量对高脂诱导的非酒精性脂肪肝病（ NAFLD）的影响。对成年SD雄性大鼠随机分为正常组（ CON）和高脂饮食诱导NAFLD组（ HF），HF组下分HFC0、HFC1、HFC2和HFC5组，分别对应饲料中添加0％、1％、2％和5％的胆固醇，各组于饲养4周和8周结束时断头取血，检测血清TC、TG含量和ALT、AST活性，对8周的肝组织切片HE染色。结果显示与对照组相比，随着胆固醇摄入量增加，饲喂8周的大鼠体重、肝重及肝比重增加明显；肝形态改变明显，细胞脂质聚积和炎性浸润显著；HFC2、HFC5组于4周时血TC含量、ALT及AST升高显著。研究结果表明：高胆固醇饮食可加重肝脂质聚积、肝功能异常，并促进了非酒精性脂肪肝病的发展。
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