不同氮肥对番茄根际土壤真菌多样性的影响

摘要

真菌是土壤生物量的重要组成部分,在农业生态系统中起着重要的作用.利用18S rDNA的PCR - DGGE图谱分析,对不同氮素形态(NO3-、NH4+、Gly)下土壤中真菌群落的多样性进行比较.结果表明,施用不同形态有机氮肥的土壤真菌多样性从高到低排序为,施Gly＞施NH4Cl＞施KNO3＞未施肥的土壤.聚类分析显示,所测序的真菌大部分属于子囊菌纲(Ascomycota),占真菌总数的28.125％,其次为担子菌纲(Basidiomycota),占真菌总数的21.875％.研究结果为番茄生产中施用不同形态的氮肥提供理论依据.%As an important part of soil biomass,fungi play a very critical role in agricultural ecosystem.Through PCR-DGGE of 18S rDNA,the fungal diversity of rhizosphere soil of tomato cultivation under different forms of nitrogen application,namely NO3-,NH4+and Gly were compared.The results showed that,with the application of different nitrogen forms,the fungal diversity decreased in the order of Gly,NH4Cl and KNO,respectively.By cluster analysis,it was found that most of the fungi belonging to Ascomycota,which accounted for 28.125 % of the total,next came Basidiomycota,which accounted for 21.875 %.