巴山榧树及近缘种的 psbA-trnH 序列分析

摘要

采用PCR产物直接测序法对巴山榧树12个地理种群的叶绿体psbA-trnH序列进行了测定，并从Gen Bank搜索并下载巴山榧树近缘种的psbA-trnH序列，运用Clustal X和MEGA 4.1软件分析和构建系统发育树。结果表明，巴山榧树12个地理种群间的遗传分化程度较低；巴山榧树与云南榧、日本榧树的亲缘关系较近，而与长叶榧、榧树、加州榧和佛罗里达榧的亲缘关系较远。研究结果为进一步探讨巴山榧树分子谱系地理学及榧树属的分子系统发育提供了理论依据。%Chloroplast psbA-trnH intergenic spacer sequences of 12 geographical populations of Torreya fargesii were sequenced by using PCR products sequencing method.psbA-trnH sequences of its closely related species were retrieved from GenBank.Clustal X and MEGA 4.1 were used to analyze the psbA-trnH sequences among these geo-graphical populations of T.fargesii and its closely related species, and to construct its phylogenetic tree.The re-sults showed that genetic differentiation among 12 geographical populations of T.fargesii was low.T.fargesii had close corelation with T.fargesii var.yunnanensis and T.nucifera, but had minor corelation with T.jackii, T.grandis, T.californica and T.taxifolia.The results of this research could provide theoretical foundation for fur-ther investigation on molecular phylogeography of T.fargesii and phylogeny of Torreya.