目的:探讨转录因子SOX7对前列腺癌PC-3细胞增殖、细胞周期、迁移和侵袭能力的影响及其机制.方法:采用脂质体法转染高表达SOX7重组质粒(pCDNA3.1-SOX7),以转染空质粒(Vector)作为对照,检测SOX7对PC-3细胞株增殖、细胞周期、迁移及侵袭的影响,并用Western blot法检测相关蛋白表达水平.结果:pCDNA3.1-SOX7显著抑制PC-3细胞增殖能力,并且诱导细胞周期发生G1期阻滞,同时,使Cylcin D1、E表达量明显下调.此外,pCDNA3.1-SOX7转染后,PC-3细胞迁移距离显著减少,穿透基质胶的细胞数量也显著降低,并且伴随着间质表型标记物MMP-2、MMP-9和N-cadherin蛋白表达减少,上皮标记物E-cadherin蛋白表达增加.结论:SOX7抑制前列腺癌PC-3细胞的增殖、周期、迁移及侵袭能力,其机制可能与调控这些细胞行为的相关蛋白表达有关.%Objective: To investigate the effects and mechanisms of transcription factor SOX7 on PC-3 cells proliferation, cell cycle, cell migration and invasion. Methods: PC-3 cells were transfected with high-expressed SOX7 recombinant plasmid (pCDNA3.1-SOX7) and further detected the changes of cell proliferation, cell cycle, migration, invasion, and related protein expressions. Results: After transfection with pCDNA3.1-SOX7, significant inhibition effects was shown in the PC-3 cell proliferation and G1/S cell cycle arrest, which were accompanied with decreased expressions of cyclin D1 and E. Furthermore, transfection of pCDNA3.1-SOX7 markedly decreased the cell migration distances and invasion capacity, with a reduction of the mesenchy-mal markers (MMP-2, MMP-9 and N-cadherin), however, the epithelial marker E-cadherin was increased. Con-clusion: SOX7 inhibits PC-3 cell proliferation, cell cycle, migration and invasion through regulation of its related protein expressions.
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