催吐萝芙木离体培养和植株再生体系的建立

摘要

为建立催吐萝芙木 (Rauvolfia vomitoria Afzel.) 的快繁再生体系, 以茎段为外植体, 比较了植物生长调节剂对其愈伤组织诱导、分化及生根的影响.结果表明, 诱导愈伤组织的适宜培养基为MS+2, 4-D 1.0 mg L–1+TDZ 0.5 mg L–1或MS+2, 4-D2.0 mg L–1+TDZ 0.5 mg L–1, 出愈率达100％且生长状况良好;诱导丛生芽的最佳培养基为MS+6-BA 3.0 mg L–1+NAA 0.1 mg L–1, 出芽率为46.6％, 平均出芽数为3.04.这为催吐萝芙木的快速繁殖和遗传转化研究奠定了基础.%To constructe the rapid regeneration system of Rauvolfia vomitoria Afzel., the effects of plant growth regulators on callus induction, differentiation, and rooting were studied with young stems as explants. The results showed that the optimal medium for callus induction was MS+2, 4-D 1.0 mg L–1+TDZ 0.5 mg L–1 or MS+2, 4-D 2.0 mg L–1+TDZ 0.5 mg L–1 with callus induction rate of 100％. The optimal medium for callus differentiation was MS+6-BA 3.0 mg L–1+NAA 0.1 mg L–1 with shooting rate of 46.6％ and average buds of 3.04. So, these would lay a foundation for studying on rapid regeneration and genetic transformation of R. vomitoria.