香蕉果实冷胁迫相关MaWRKY11转录因子的特性、互作蛋白筛选与鉴定

摘要

为探讨香蕉(Musa acuminata)响应冷胁迫的分子机制，从香蕉果实冷害的数字基因表达谱中筛选并分离了1个WRKY转录因子，命名为MaWRKY11。MaWRKY11具有2个WRKY保守结构域，属于I类WRKY成员，定位于细胞核，是核蛋白。MaWRKY11具有转录激活活性，且激活区在N端。实时荧光定量PCR分析表明MaWRKY11受冷胁迫诱导，外源茉莉酸甲酯(MeJA)处理减轻香蕉果实冷害的同时也上调了其表达。另外，酵母双杂交筛选表明，MaWRKY11可与脱水诱导的早期应答蛋白MaERD相互作用。这些表明MaWRKY11可能通过与逆境相关蛋白如MaERD互作来响应香蕉果实的冷胁迫。%In order to understand the molecular mechanism, oneWRKY transcription factor, named asMaWRKY11, was isolated and identiifed from Digital Gene Expression Data (DGE) of banana (Musa acuminata) fruits. The results showed that MaWRKY11 contained two WRKY conserved domains, belonging to Group I of WRKY family, and located in nucleus as a nuclear protein. The MaWRKY11 possessed transcriptional activity in yeast cells and the transcriptional domain located at its N-terminus. Moreover, real-time quantitative PCR displayed thatMaWRKY11 was induced by cold stress, and chilling injury of banana fruits reduced and the expression of MaWRKY11 was up-regulated treated by MeJA. In addition, C-terminus of MaWRKY11 was used as a bait to screen its potential interacting proteins by using yeast two-hybrid system, and it was found that MaWRKY11 could interact with early responsive to dehydration protein, MaERD, which was also induced by cold. So, it was suggested that MaWRKY11 might respond to chilling stress through interacting with stress-related proteins, such as MaERD.