角果木内生真菌Cladosporium cladosporioides JG-12次生代谢产物及其生物活性

摘要

The secondary metabolites from the endophytic fungus Cladosporium cladosporioides JG-12 of Ceriops tagal were isolated and purified by various column chromatographic techniques.The structures of the compounds were identified through a combined analysis of physicochemical properties and spectroscopic evidence.The compounds were tested to observed their anti-bacterial,anti-acetylcholinesterase activities and their inhibitory activity against Panagrellus redivivus in vitro.Six compounds were isolated from the endophytic fungus Cladosporium cladosporioides JG-12 of Ceriops tagal,and their structures were determined as (5 S)-5-hydroxy-7-(4"-hydroxy-3"-methoxy-phenyl)-1-phenyl-3-heptanone (1),ilicicolin H (2),2-chloro-3,5-dimethoxybenzyl alcohol (3),(7R)-methoxypurpuride (4),(5aS,9S,9aS)-1,3,4,5,5a,6,7,8,9,9a-decahydro-6,6,9a-trimethyl-3-oxonaphtho [1,2-c] furan-9-yl N-acetyl-L-valinate (5),ergosterolperoxide (3β3-hydroxy-5,8-epidioxyergosta-6,22-diene) (6).The Compound 1 showed weak inhibitory effect against Ralstonia solanacearum and Staphylococcus aureus,and the Compound 3 exhibited inhibitory effect against Candida albicans.The Compounds 1 and 3-5 displayed their inhibitory activities against Panagrellus redivivus.The Compounds 1-5 revealed their inhibitory activities against acetylcholinesterase.%采用多种柱色谱技术对角果木内生真菌Cladosporium cladosporioides JG-12的次生代谢产物进行分离纯化,通过波谱数据分析,结合理化常数对化合物的结构进行鉴定,同时,对化合物的抗菌活性、乙酰胆碱酯酶抑制活性以及全齿复活线虫(Panagrellus redivivus)抑制活性进行测试.结果表明,从角果木内生真菌Cladosporium cladosporioides JG-12的次生代谢产物中分离鉴定了6个化合物,分别为:(5S)-5-hydroxy-7-(4”-hydroxy-3”-methoxy-phenyl)-1-phenyl-3-heptanone(1),ilicicolin H(2),2-chloro-3,5-dimethoxybenzyl alcohol (3),(7R)-methoxypurpuride (4),(5aS,9S,9aS)-1,3,4,5,5a,6,7,8,9,9a-decahydro-6,6,9a-trimethyl-3-oxonaphtho[1,2-c] furan-9-yl N-acetyl-L-valinate(5),ergosterolperoxide(3β-hydroxy-5,8-epidioxy-ergosta-6,22-diene) (6).其中,化合物1具有抗青枯雷尔氏菌(Ralstonia solanacearum)和金黄色葡萄球菌(Staphylococcus aureus)的活性,化合物3具有抗白色念珠菌(Candida albicans)的活性;化合物1和3～5具有全齿复活线虫抑制活性;化合物1～5具有乙酰胆碱酯酶抑制活性.