红树植物角果木内生真菌Penicillium sp.J54的次生代谢产物研究

摘要

目的 红树植物角果木内生真菌Penicillium sp.J54大米固体培养基发酵的次生代谢产物的研究.方法 采用多种色谱技术进行分离纯化,并根据化合物的理化常数、核磁和质谱数据进行结构鉴定;通过滤纸片琼脂扩散法进行体外抗菌活性测试;通过Ellman比色法进行乙酰胆碱酯酶抑制活性测试.结果 从Penicillium sp.J54的大米固体培养基发酵产物中分离得到了6个化合物,分别鉴定为methyl 2-hydroxy-2-(5-hydroxy-2,7-dimethyl-4-oxo-4H-chromen-3-yl)acetate(1)、coniochaetone B(2)、dihydrocitrinone(3)、asperbiphenyl(4)、erythro-11 α-hydroxyneocyclocitrinol(5)和麦角甾-5,7,22-三烯-3β-醇(6).活性测试结果表明化合物3,4和6对烟草青枯病原菌具有微弱的抑制活性,而化合物2则对乙酰胆碱酯酶有较强的抑制活性.结论 化合物1为新化合物,4为首次从青霉菌中分离得到.%Objective To study the secondary metabolites from the endophytic fungus Penicillium sp.J54 cultivated on solid rice medium.Methods The compounds were isolated by various column chromatographic techniques,and their structures were identified through a combined analysis of physicochemical properties,as well as by NMR and MS data.The in vitro antimicrobial activity and acetylcholinesterase inhibitory activity of these compounds were evaluated by paper disc diffusion and Ellman colorinetric methods,respectively.Results Six compounds were isolated from the endophytic fungus Penicillium sp.J54 cultivated on solid rice medium,and their structures were determined as methyl 2-hydroxy-2-(5-hydroxy-2,7-dimethyl-4-oxo-4H-chromen-3-yl)acetate(1),coniochaetone B(2),dihydrocitrinone(3),asperbiphenyl(4),erythro-1 1α-hydroxyneocyclocitrinol(5),and ergosta5,7,22-triene-3β-ol(6).The bioactivity results showed that compounds 3,4 and 6 presented weak inhibition against Ralstonia solanaceanum,and 2 revealed strong inhibition against acetylcholinesterase.Conclusion Compound 1 was a new compound,and 4 was reported for the first time from Penicillium sp.