核心蛋白聚糖对骨性关节炎中软骨修复作用的形态学研究

摘要

Objective To investigate the effect of decorin for cartilage repair in osteoarthritis using the morphological method. Methods Decorin and transforming growth factor ( TGF) were injected into articular cavity. General observation and histological analysis were performed. Using the rat model, the effect of decorin for cartilage repair was investigated through International Cartilage Repair Society ( ICRS ) scoring system. Results After two weeks of decorin injection , the surface of the decorin group was more smooth than that of the control group. After four weeks of decorin injection, each side of the surface, matrix, cell distribution, cell population, subchondral bone and cartilage mineralization in the decorin group were significantly better than those of the control group( P ＜0.05) . Conclusion Decorin has good effect in treating cartilage repair and the mechanism needs to be further studied.%目的 利用形态学方法,初步探讨核心蛋白聚糖(decorin,DCN)对骨性关节炎中软骨修复的作用.方法 选取SD大鼠制作骨关节炎模型,将DCN和转化生长因子(TGF)注射进关节腔,通过大体观察和组织学观察,利用国际软骨修复协会(International Cartilage Repair Society,ICRS)评分研究DCN对骨关节炎中软骨缺损的修复效果.结果 注射DCN 2周后实验侧比对照侧关节软骨表面较光滑连续,但在基质、细胞分布、软骨下骨、软骨矿化方面的差异ICRS评分均无显著统计学意义.4周后实验侧比对照侧,关节软骨表面更光滑连续,基质更透明,细胞分布成簇较少,软骨下骨重塑好,各项ICRS评分差异均有统计学意义,但软骨矿化的差异ICRS评分无显著统计学意义.结论 DCN对骨关节炎中软骨的修复有一定效果,但其机制需要进一步研究.