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in kunstlisches recombinant substrate ragg - 1 and the native aggrecan study the proteolytic effect of aggrecanase in cell systems
in kunstlisches recombinant substrate ragg - 1 and the native aggrecan study the proteolytic effect of aggrecanase in cell systems
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机译:在重组底物ragg-1和天然蛋白聚糖中研究蛋白聚糖酶在细胞系统中的蛋白水解作用。
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摘要
The invention refers to a recombinant substrate for Aggrecanase in vitro testing systems containing a group of structural elements comprising the signal sequence of CD5, the FLAG-epitope for M1 monoclonal antibody detection, the interglobular domain of human aggrecan, the hinge region of human IgG1, the CH2 region of human IgG1 and the CH3 region of human IgG1; a DNA encoding the recombinant substrate; vectors and host cells containing said DNA and use of the recombinant substrate to study the activity of aggrecanase to detect new enzymatic cleavage sites, to purify aggrecanase chromatographically, to clone the aggrecanase cDNA by functional cloning, to screen for an aggrecanase inhibitor; a method for monitoring the onset or progression of osteorthritis and a diagnostic aid containing the recombinant substrate. IMAGE
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