Mucin Glycan: Expression and Potential Role in Prostate Cancer Metastasis

摘要

The purpose is to identify the role of mucin-type O-glycan associated glycotopes mediated metastasis of prostate cancer cells. Significant problem in the management of prostate cancer is emergence of hormone independent cancer progression and metastasis to vital organs such as lymph node, lung, bone and brain, finally leads to death. Currently the difficulty in finding solution for prostate cancer metastasis is unavailability of in vitro cell model system which mimics clinical progression of prostate cancer. Altered cell surface glycosylation, ie increased expression of sLex antigen, is characteristics of metastatic cancers, including prostate cancer. The main objective of current application to identify the role of mucin glycan associated epitopes involved in prostate cancer metastasis and role of extended core 1 structure in prostate cancer progression. Dr. Lin's laboratory has established a cell model system using the LNCaP cell line consisting of the parental C-33, the high passage C- 81 cells and LNCaP cell derived C4-2B bone metastatic cells. The characteristics of this cell model system closely resemble the clinical progression of prostate cancer.