A generalized cost function of passenger is built with measurement indexes, including economy, speediness, convenience, comfort and security. Then a mode split model is proposed by using the disaggregate theories. In order to analyze the changes of the passenger traffic structure in middle-and-long passenger corridor, Wuhan-Guangzhou corridor is selected as an example. And the market shares of transportation modes are forecasted with different values of time. The results show that the market shares of ordinary railway are more than 45%, which means that ordinary railway is more suitable for passengers. Finally, operation strategy of high-speed railway is analyzed based on profit. Results show that, due to the competition between high-speed railway and ordinary railway, it is difficult to achieve the maximum profit for high-speed railway operator and railway department at the same time. The high-speed railway operator has the highest profit when the price and running speed of high-speed railway are 275 yuan and 325km/h respectively;and the highest profit of railway department can be obtained only when the price and running speed of high-speed railway change to 350 yuan and 300km/h respectively.%以经济性、快速性、便捷性、舒适性及安全性为衡量指标，建立乘客出行广义费用函数；结合非集计理论和方法，构建中长距离通道客流分担率模型.并以武汉—广州客运通道为例，分析不同时间价值下客流结构的变化情况，发现普通铁路更加符合旅客的出行偏好，其客运分担率高于45%.最后，以利润为指标，对高速铁路运营策略进行了分析.研究结果表明，由于普通铁路与高速铁路之间的竞争关系，高速铁路运营利润及铁路总利润难以同时达到最优；当以高速铁路运营利润最大为目标时，高速铁路票价和旅行速度分别为275元和325km/h；当以铁路总利润最大为目标时，高速铁路票价和旅行速度分别为350元和300km/h.
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