Objective To establish an acetylcholine-induced atrial fibrillation (AF) model in the isolated rabbit heart. Methods Forty healthy male New Zealand rabbits (2.2-2.5 kg) were used in the study. The isolated rabbit hearts were randomly assigned to normal perfusion (group A), normal perfusion with burst high-frequency electric stimulation (group B), acetylcholine perfusion (group C) and acetylcholine perfusion with burst electric stimulation (group D) (n = 10 in each group). The isolated hearts were perfused with Langendorff perfusion system. Results The AF incidence in group D ( acetylcholine perfusion combined with burst electric stimulation) was 50% ; while no AF was induced in other three groups. Conclusion A simple and stable acetylcholine-induced atrial fibrillation model has been established in isolated rabbit heart with Langendorff perfusion system.%目的 建立乙酰胆碱诱导的离体Langendorff灌流的兔的心房颤动模型.方法 健康雄性的新西兰兔40只,2.2 ～2.5 kg,分为4组,分别为正常灌流组、正常灌流+burst刺激组、正常灌流+乙酰胆碱灌注组和正常灌流+乙酰胆碱灌注+ burst刺激组,每组各10只.在Langendorff灌流装置下行主动脉逆行灌流,通过乙酰胆碱灌注和burst刺激诱发心房颤动.结果 正常灌流组、正常灌流+burst刺激组和正常灌流+乙酰胆碱灌注组均没有发生心房颤动,而正常灌流+乙酰胆碱灌注+ burst刺激组心房颤动的发生率为50％.结论 在Langendorff心脏灌流时,给予乙酰胆碱灌注模拟胆碱能激活,并给予burst刺激模拟应激事件,可以稳定地诱导心房颤动的发生.
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