分子印迹传感器对标本中塑化剂直接快速检测

摘要

An electrochemical sensor based on molecularly imprinted sensor for quick detection of DINP was developed in this research.Firstly,imprinted polymer particles(MIPs)were prepared through thermal polymerization.Then,MIPs and agarose were mixed in proportion.The electrochemical sensor for DINP was fixed by the mixture on a glassy carbon elec-trode.The sensor can be combined with the template molecule DINP and its electrochemical signals are influenced by DINP concentration.Thus,it could be used to detect DINP levels in samples,and DINP concentration was quickly detected in wine samples without sample pretreatment.The linear equation in this study wasΔI=0.03c+3.07,the correlation coefficient is R2=0.998,the linear range was 5×10-8-1×10-6,mol/L,the sensitivity limit was 2.7×10-8,mol/L,and the recovery rate was 105.3%,-115.7%,.%本文构建了能够快速检测邻苯二甲酸二异壬酯(DINP)的分子印迹电化学传感器．首先利用分子印迹技术以热聚合的方法制备了分子印迹聚合物颗粒(MIPs)；然后将优化的MIPs与琼脂糖溶液混匀固定在玻碳电极表面制备成DINP分子印迹电化学传感器；传感器可以特异性结合模板分子DINP且其电化学信号与DINP的浓度相关．再利用传感器检测样品中DINP的含量，检测过程不需要样本前处理，可以用于现场直接检测．该方法的线性方程为ΔI＝0.03c+3.07，相关系数R2＝0.998，线性范围5×10-8～1×10-6,mol/L，检测限为2.7×10-8,mol/L，在实际样品中的加标回收率为105.3%,～115.7%,．