Direct and simultaneous assays for antigens in clinical specimens by monoclonal antibody immunoblot, antibodies for use therein and corresponding hybridomas

摘要

This invention relates to a diagnostic method of employing a monoclonal antibody in a Western immunoblot assay for detecting antigens in clinical specimens. This invention further provides a method of simultaneously detecting a variety of antigens in a single Western immunoblot assay by incorporating in said assay monoclonal antibodies to each antigen to be detected. Said method presents a differential diagnosis procedure employing a panel of monoclonal antibodies to antigens of various symptomatically related etiologic agents.;This invention further relates to a murine IgG1 monoclonal antibody which binds to a protease sensitive, periodate insensitive epitope on Mycoplasmapneumoniae membrane polypeptide with an approximate molecular weight of 43K. It also specifically relates to a diagnostic method for detecting M.pneumoniae with said monoclonal antibody in clinical specimens.