Characterization of Monoclonal Antibodies to Two Treponema denticola Serotypes bythe Indirect Fluorescent-Antibody Assay

摘要

Members of the genus Treponema have been implicated as possible aetiologic agentsof periodontal disease. Previously developed murine monoclonal antibodies (MAbs) to two Treponema denticola strains were characterized by an indirect fluorescent-antibody (IFA) technique. Fifteen T. denticola strains and 32 other bacteria commonly isolated from periodontal pockets were used in the screening process. Two monoclonal antibodies to T. denticola isolates. There was no cross-reactivity between MAbs to the two serotypes. Preliminary tests of the MAbs on human periodontal samples show that they are useful in detecting these bacteria in clinical samples.