苎麻脱胶果胶复合酶的优选及其效果分析

摘要

To develop efficient enzyme preparation used in ramie degumming, the efficient strains used in ramie bio?degumming subjected to liquid state fermentation, which were screened and accessed. The characteristic parameters of extracellular enzymes including pectinase activity, mannanase activity and protein content, etc. were further determined, and the comprehensive indexes of the ramie degumming system, such as fiber morphology, mass loss rate, residual gum rate, etc. , were analyzed. The results show that pectinase activities of the group N and the group G were 61. 79 IU/mL and 13. 69 IU/mL, respectively, and their special enzyme activities were 4. 47 IU/mg and 0. 93 IU/mg, respectively. Cellulase activities of the group N and the group G were only 0. 01 IU/mL and 0. 02 IU/mL, respectively. Their mass loss rate and residual gum rate of the group N and the group G were 20. 30%and 18. 69%, and 2. 52% and 4. 21%, respectively, which are close to those of strains in practical industry application. The single fiber fineness of the group N was 5. 31 dtex, and the fracture strength of the bundle fiber was 4. 8 cN/dtex, which belonged to high?quality degummed ramie fiber.%为研发高效苎麻脱胶酶制剂,对筛选并保藏的苎麻脱胶高效菌种进行液态发酵,测定胞外酶的果胶酶活力、甘露聚糖酶活力和蛋白质含量等特征参数.通过苎麻酶法脱胶,从纤维表观形态学、质量损失率、残胶率等方面综合分析苎麻脱胶效果.结果表明:第N组和第G组的果胶酶活力较优,分别为61.79、13.69 IU/mL,比酶活力分别为4.47、0.93 IU/mg;第N组和第G组的纤维素酶活力仅为0.01、0.02 IU/mL;第N组和第G组苎麻质量损失率分别为20.30%和18.69%,残胶率分别为2.52%和4.21%,均接近实际工业化应用水平;第N组的单纤维线密度为5.31 dtex,束纤维断裂强度为4.8 cN/dtex,属于优质脱胶苎麻纤维.