基于转录组测序对茶树 GST 基因表达的研究

摘要

Glutathione S-transferases (GSTs) belong to a superfamily of multifunctional enzymes and are ubiquitous in plants. Forty nine CsGSTs genes were identified by transcriptome analysis of Zhonghuang 2 and Longjing 43 under control and shading treatment. Nineteen CsGSTs with relatively high expression levels in buds were used for sequence and phylogenetic tree analysis. Real-time fluorescence quantitative PCR (qRT-PCR) was performed to analyze the expression patterns of 8 candidate genes in different leaf positions of Longjing 43. The results showed that CsGSTs were expressed in all tested leaves, but exhibited different expression patterns. CsGST20 showed an increasing expression from a bud with a leaf to the sixth leaf, suggesting a potential role in stress resistance. On the other hand, the expression level of CsGST24 dropped significantly, suggesting that CsGST24 might participate in anthocyanin accumulation in tea plants.%谷胱甘肽转移酶（Glutathione S-transferases, GSTs）在植物体内普遍存在，是一类具有多种生物学功能的超家族蛋白酶。本研究通过对中黄2号、龙井43在正常光照和遮荫处理下的转录组测序，筛选获得了49个 CsGSTs 基因家族成员，并对其中19个在芽叶中表达量较高的 CsGSTs 基因进行了序列分析和聚类分析。另外对表达量较高的8个候选基因进行荧光定量表达分析，研究它们在龙井43不同叶位中的表达模式。结果表明这些 CsGSTs 基因在一芽一叶到第六叶中均有表达，但各自呈现出不同的变化规律。CsGST20在龙井43一芽一叶到第六叶中的表达量逐渐上升，可能与植物抗胁迫有关，而 CsGST24的表达量则显著下降，可能与花青素代谢有关。