保护品种云茶1号茶树全长转录组测序分析

摘要

为探讨云茶1号茶树品种优异性状的遗传基础,采用 PacBio 平台进行全长转录组测序,最终获得Polished consensus序列213389个,预测到CDS有223120个,检测到195062个SSR位点.在NR数据库有170264个同源序列比对到980个物种;有103124个在KOG数据库得到注释,根据其功能各分为26类;有65524个得到GO注释分为细胞组分、分子功能及生物学过程等三大类的55个功能组;根据KEGG数据库, 105972个得到了注释,涉及到216个代谢途径分支,包括茶叶品质、活性物质代谢以及抗逆等相关基因等;还预测到隶属于60个转录因子家族的转录因子有5785个.这些结果为进一步开展云茶1号茶树特异性状基因的标记性引物开发、遗传研究以及品质形成和抗逆机制研究奠定了基础.%To explore the genetic basis for important traits,the full-length transcriptome of the'Yuncha 1'(Camellia assamica)was sequenced by using PacBio Platform. A total of 213 389 polished consensus were generated,223 120 coding sequences were predicted and annotated, and 195 062 SSR loci were found. According to NR databases, 170 264 homologous sequences were mapped to 980 species, 103 124 unigenes were further annotated and grouped into 26 functional categories in KOG databases, 65 524 unigenes were annotated against GO database and divided into cellular component, molecular function and biological process categories with a total of 55 functional groups. KEGG pathway analysis showed that 105 972 unigenes could be broadly classified into 216 metabolism pathways according to their function, and some of them were involved in quality, bioactive substances, and resistance gene, etc. It is also predicted that there were 5 785 transcription factors belonging to 60 transcription factor families. The experimental results will give important data for development of SSRs of specific traits, genetic analysis and studies involved in quality formation and resistence mechanism in tea cultivar 'Yuncha 1'.