茶黄素对高糖诱导的人晶状体上皮细胞氧化损伤的影响

摘要

研究茶黄素（Theaflavin，TF）对高糖(High glucose，HG)诱导的人晶状体上皮细胞（Human lens epithelial cells，HLECs）氧化损伤的影响。采用体外培养人晶状体上皮细胞，用葡萄糖诱导细胞成氧化损伤模型，外加一定浓度的茶黄素干预，比色法检测细胞体内SOD、CAT、GSH-Px、MDA活力和含量的变化。结果表明相对于正常培养的细胞，高糖诱导细胞体内SOD、CAT、GSH的活性降低，MDA含量上升；加入茶黄素后，提高了细胞内SOD、CAT、GSH-Px的活力，降低了细胞内MDA的含量，与模型组细胞相比，具有显著差异（P＜0.01）。%To study the effect of theaflavins on oxidative damage in human lens epithelial cells under high glucose. Methods:Human lens epithelial cells were cultured with HG (30mM)to Establish the oxidative damage model. After the culture under suitable temperature for 24h ,32μM and 64μM TF were added into TF-growps. The SOD、CAT、GSH-Px、MDA were measured with using colorimetry. Results:Compared with the normal cultured cells, The SOD, CAT, GSH-Px activity were redused in cells induced by high glucose, the MDA content increased;After add the TF, it improved the SOD, CAT, GSH activity, MDA content decreased(P<0.01). Conclusion: Theaflavins can effectively control the activities of antioxidant enzymes in human lens epithelial cells induced by high glucose .