香梨优斑螟几丁质合成酶(CHS-A)基因片段序列分析

摘要

几丁质合成酶A作为昆虫蜕皮和变态发育过程中几丁质合成的关键酶,已成为环境友好杀虫剂的理想靶标之一.为研究香梨优斑螟几丁质合成酶的基因结构及潜在应用,以同源基因克隆技术获得目的基因序列,利用生物信息软件进行序列分析.结果显示:该基因5'端2534bp碱基序列编码了蛋白质804个氨基酸,包含催化区域11个保守区中的8个,TMHMM 2.0软件预测此蛋白质中含有9个跨膜螺旋.以13种昆虫氨基酸序列(相似度大于90％)构建系统发育树,结果显示香梨优斑螟(Euzophera.pryiella)与脐橙螟蛾(Amyelois Transitella)进化关系最近,其次云杉卷叶蛾(Choristoneura fumiferana),与三种蝶类(Papilio sp.)及家蚕(Bombyx mori)进化关系较远.%Insect chitin biosynthesis is the essential enzyme that to form chitin and to meet the needs of insect molting.As chitin is absent in higher animals, the pathway of insect chitin biosynthesis has attracted much interest in serving as a potential target for developing safe and effective insecticides.In order to study the gene structure and its potential application, chitin synthase gene of Euzophera pryiella, EpCHS-A, was cloned by homology gene cloning techniques.The fragment of the 5'-end EpCHS-A gene contained 2534bp, encoding 804 amino acids, the 8 of 11 conserved domains was found at the amino acid sequence level.TMHMM 2.0 software was used to predict transmembrane structure that it had 9 transmembrane structures.Based on the amino acid sequences of 13 species insects (more than 90％ similarity) to construct phylogenetic tree, the results showed the distance was very close to Euzopher Pryiella and Amyelois Transitella, and closer to Choristoneura fumiferana, but was farther to 3 kind of Papilio sp.and Bombyx mori.