Methods for the identification of inhibitors of chitin synthase 2, s-adenosylmethionine decarboxylase, putrescine aminopropyltransferase, and methylenetetrahydrofolate reductase as antibiotics

摘要

The present inventors have discovered that Chitin Synthase 2, S-Adenosylmethionine Decarboxylase, Putrescine Aminopropyltransferase, and Methylenetetrahydrofolate Reductase are essential for normal rice blast pathogenicity and are, thus, useful as targets for the identification of antibiotics, preferably fungicides. Accordingly, the present invention provides methods for the identification of compounds that inhibit Chitin Synthase 2, S-Adenosyl-methionine Decarboxylase, Putrescine Aminopropyltransferase, and Methylenetetrahydrofolate Reductase expression or activity. The methods of the invention are useful for the identification of antibiotics, preferably antifungals.