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CHITIN SYNTHASE GENE CHS3B OF FUSARIUM AND USES THEREOF

机译:镰刀菌的几丁质合成酶基因CHS3B及其用途

摘要

A chitin synthase gene Chs3b of a fusarium, a nucleotide sequence of which is represented by SEQ ID NO:1, and a coded amino acid sequence of which is represented by SEQ ID NO:2. For five different gene segments (named as Chs3b-1, 2, 3, 4 and 5) of the Chs3b gene, five different RNAi carriers are constructed and are used to convert the fusarium, and it is found that the growth, development and pathogenicity of the fusarium are obviously constrained. By expressing RNAi carriers for a Chs3b gene in a plant, the invasion of a fusarium can be constrained, and scab resistance capabilities of a transgenic plant can be improved.
机译:镰刀菌的几丁质合酶基因Chs3b,其核苷酸序列由SEQ ID NO:1表示,并且其编码的氨基酸序列由SEQ ID NO:2表示。对于Chs3b基因的五个不同基因片段(分别称为Chs3b-1、2、3、4和5),构建了五个不同的RNAi载体并用于转化镰刀菌,发现其生长,发育和致病性镰刀菌明显受到限制。通过在植物中表达Chs3b基因的RNAi载体,可以抑制镰刀菌的入侵,并可以提高转基因植物的抗sc疮能力。

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