青枯菌对木麻黄防御酶活性及可溶性蛋白含量的影响

摘要

To study the responses of Casuarinaceae equisetifolia defense enzymes to Ralstonia solanacearum, the contents of soluble protein and activities of peroxidas ( POD) , catalase ( CAT) , superoxide dismutase ( SOD) , polyphenol oxidase ( PPO) and phenylalanine ammonia lyase ( PAL) were measured after R. solanacearum inocula-ted on C. equisetifolia by the injured root inoculation of potted seedling method. The result showed that the activity of protective enzymes in C. equisetifolia had been changed significantly after inoculation and the activity of SOD, PPO and PAL showed a waved curve. However, the activity of CAT and POD were decreased and much lower than that in the control. In addition, the contents of soluble protein were increased, which were consistent with the changes of SOD, PPO and PAL activities. The results indicate that R. solanacearum may induce resistance by increasing the activities of SOD, PPO and PAL in C. equisetifolia leaves.%采用移栽浸根法将青枯菌接种到木麻黄A8无性系上,研究接种后木麻黄叶片中超氧化物歧化酶(SOD)、 过氧化氢酶(CAT)、 过氧化物酶(POD)、 多酚氧化酶(PPO)和苯丙氨酸解氨酶(PAL)等防御酶活性及可溶性蛋白含量的变化规律.结果表明:接种后木麻黄防御酶活性发生了显著变化,青枯菌诱导了SOD、P P O、P AL酶活性的增加,表现出"升—降—升—降"的变化趋势,在木麻黄枯萎前,酶活性下降.相反,青枯菌抑制了木麻黄CAT、POD的活性,酶活性明显低于对照处理.此外,可溶性蛋白的含量也增加,其含量变化与SOD、P P O和P AL酶活性变化趋势基本一致,青枯菌可诱导提高木麻黄叶片SOD、P P O和P AL防御酶活性抵抗病菌的侵害.