COX-2抑制剂塞来昔布对人舌鳞癌Tca8113细胞迁移的抑制作用

摘要

目的 研究环氧化酶-2(COX-2)抑制剂塞来昔布(Celecoxib)抑制人舌鳞癌Tca8113细胞迁移的作用.方法 采用细胞划痕实验、细胞.基质黏附实验和Boyden趋化小室测定Tca8113细胞的迁移能力.观察塞来昔布对Tca8113细胞迁移作用的影响.结果 10 μmol/L和20 μmol/L塞来昔布处理细胞24 h后.细胞迁移数与对照组相比减少有统计学意义(P<0.05),Tca8113细胞的迁移能力显著减弱.不同浓度塞来昔布处理Tca8113细胞24 h后,其与包被Fn基质胶表面上的黏附情况呈剂量依赖天系,随塞来昔布浓度的增加,Tca8113细胞在基质表面的黏附显著降低.结论 COX-2抑制剂塞来昔布具有抑制Tca8113细胞迁移的能力,其机制有待进一步研究.%Objective To investigate the role of COX-2 inhibitor celecoxib in inhibiting the migration of human tongue squamous cell carcinoma Tca8113 cells. Methods The effects of celecoxib on Tca8113 cell migration were tested using scrape motility assay, cell-matrix adhesion assay and Boyden chamber motility assay. Results Following a 24-hour incubation with 10 and 20 μmol/L celecoxib, the migration of Tca8113 cells was significantly decreased (P<0.05). Celecoxib treatment for 24 h also resulted in significantly decreased adhesion of Tca8113 cells on Fn-coated surface in a dose-dependent manner. Conclusion COX-2 inhibitor celecoxib can inhibit Tca8113 cell migration, the mechanism of which awaits further investigation.