小口径聚氨酯人工血管的制备及其力学性能、细胞相容性评价

摘要

Objective To develop a small-caliber vascular grafts and study its morphologies, mechanical properties and biocompatibility. Methods The effects of electrospinning conditions on the microstructure and porosity of the resulting scaffolds were investigated for preparation of a small-caliber (4 mm) polyurethane vascular grafts with optimum microstructures and mechanical properties. The mechanical properties and biocompatibility of the prepared grafts were evaluated. Results The polyurethane vascular grafts showed a three-dimensional reticular structure consisting of nanofibers, with an average porosity of (51.48±4.47)% and tensile strength of 5.85 ± 0.62 Mpa. The grafts provided a better long-term support than e-PTFE graft for endothelial cell growth and endothelialization. Conclusion The polyurethane vascular prosthesis possessed favorable microstructures, excellent mechanical properties and good biocompatibility for potential clinical application.%目的 使用静电纺丝法构建一种小口径聚氨酯血管替代物,观察人工血管的微观结构,并检测其力学性能和细胞相容性.方法 以聚氨酯为原料,通过静电纺丝法,以高速旋转的转轴为收集装置,制备4mm口径的PU人工血管.探讨纺丝液质量分数对纤维直径的影响,滚轴转速对纤维排列的影响,以及质量分数、纤维取向、管壁厚度对人工血管孔隙率的影响,并检测人工血管的力学性能及细胞相容性.结果 静电纺丝法制备的PU人工血管具有由多层微纳级超细纤维叠加而成的三维多孔网状结构,平均孔隙率为(51.48±4.47)％,轴向抗拉强度为(5.85±0.62) Mpa,无细胞毒性,并有利于内皮细胞黏附及增殖.结论 使用静电纺丝法制备小口径聚氨酯人工血管是可行的,具有潜在的临床应用前景.