目的 探讨OLETF大鼠内脏脂肪沉积与脂肪组织脂联素(APN)mRNA水平的关系.方法 自发性2型糖尿病大鼠OLETF鼠20只,同系健康对照LETO鼠10只作为正常对照组.监测体质量,定期口服葡萄糖耐量试验(OGTT),于8、32和40周分批处死大鼠,检测血糖(BG)、胰岛素、血清甘油三酯(TG)、游离脂肪酸(FFA)、胆固醇(CHOL)和血浆APN水平.分离并称量腹部脂肪,并检测脂肪组织APN的mRNA水平.结果 随病程进展,OLETF鼠体质量和内脏脂肪/体质量逐渐高于LETO组(P＜0.05),并表现出糖耐量受损.血浆胰岛素、TG、FFA、CHOL显著高于LETO鼠(p＜0.05),血浆APN水平不同周龄均显著低于LETO组(P＜0.05).脂肪组织APN的mRNA水平8周龄与LETO组无显著差异(P＞0.05),在32和40周龄显著降低(P＜0.01).结论 血浆APN水平可较好地反映OLETF鼠胰岛素抵抗和内脏脂肪含量.内脏脂肪APN转录水平降低不是初期血浆APN降低的主要原因.%Objective To investigate the relationship between visceral fat depot and adiponectin level in OLETF rats. Methods Twenty male OLETF rats and 10 male Long-Evans Tokushima Otsuka (LETO) rats were subjected to regular oral glucose tolerance test (OGTT). The rats were sacrificed at the ages of 8, 32 and 40 weeks for measurements of the body weight, blood glucose, blood lipid level, blood insulin, and weight of the visceral fat. Results Compared with LETO rats, OLETF rats had significantly higher body weight and visceral fat with impaired glucose tolerance (P<0.05). OLETF rats also had higher blood insulin, TG, FFA and CHOL levels (P<0.05). The plasma adiponectin level was significantly lower in OLETF rats than in LETO rats at different ages (P0.05). The adiponectin mRNA level in the adipose tissue of OLETF rats was comparable with that in LETO rats, but significantly decreased at 32 and 40 weeks of age (PO.01). Conclusion Plasma adiponectin level is significantly correlated to insulin sensitivity and visceral fat depots in OLETF rats, but a lowered APN mRNA expression level is not the main reason for a decreased plasma adiponectin level in the early stage.
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