目的 探讨三仁汤改善脾胃湿热证大鼠免疫功能的作用机理.方法 将50只雄性SPF级SD大鼠随机分为正常对照组、脾胃湿热模型组、三仁汤(高、中、低剂量)组,观察大鼠体质量、肛温等形态学改变,检测血清γ-干扰素(IFN-γ)和白细胞介素-4(IL-4)表达水平,取大鼠胃、十二指肠和结肠组织行病理切片,HE染色观察胃肠道炎症情况.结果 与正常对照组比较,模型组大鼠肛温升高,饮水量和摄食量减少,病理提示胃肠道存在炎症损伤,血清IFN-γ和IL-4显著增高(P＜0.01),IFN-γ/IL-4比值有升高趋势(P＞0.05).三仁汤高、中、低剂量组对脾胃湿热证大鼠的形态改变有恢复作用；中、低剂量组能降低湿热证大鼠IFN-γ、IL-4水平(P＜0.01)；高剂量组能降低IL-4水平(P＜0.01),有降低IFN-γ水平的趋势(P＞0.05),IFN-γ/IL-4比值反而升高(P＜0.05).结论 脾胃湿热证大鼠细胞免疫和体液免疫均呈亢进状态,Th1/Th2平衡有向Th1反应漂移的趋势,三仁汤能调节脾胃湿热证大鼠的免疫功能,保护胃肠道粘膜.%Objective To investigate the effects of Sanren decoction on the immune function of rats with spleen-stomach damp-heat (DHSS) syndrome. Methods Fifty male SD rats were randomly allocated into normal control group, DHSS model group, and 3 Sanren decoction groups (high, medium and low doses). The effects of the decoction on the body mass, rectal temperature (RT), water and food intake, histopathological changes of the gastrointestinal mucosa and serum levels of interleukin (IL)-4 and interferon (IFN)-y were evaluated. Results The serum levels of IFN-γ and IL-4 in the model group significantly increased compared with those in the control group (P<0.01), with a slightly increased IFN-γ/IL-4 ratio (P>0.05). Sanren decoction obviously reduced the rectal temperature and significantly decreased the production of both cytokines. High-dose Sanren decoction caused more markedly decreased IL-4 level (P<0.05) to result in a significantly increased IFN-γ/ IL-4 ratio (P<0.05). Conclusion A shift of Thl/Th2 balance toward Th1 immune response is demonstrated in rats with DHSS syndrome, and Sanren decoction produces a protective effect on the gastrointestinal mucosa by immunoregulation.
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