食管鳞状细胞癌中EZH2的表达对患者术后预后的影响

摘要

目的 通过研究鳞状细胞癌EZH2蛋白的表达,探讨其表达程度高低与患者术后预后的关系.方法 对1996～2006年本院心胸外科食管鳞癌术后病人随访,纳入研究对象,采用免疫组化方法研究其中鳞癌组织(102例)、癌旁组织(30例)、正常组织(30例)中EZH2蛋白的表达情况,研究该蛋白表达与临床病理参数(性别、年龄、分化、分期、淋巴结转移)的关系；结合随访资料,采用生存分析研究蛋白表达高低对术后患者预后的影响.结果 食管鳞状细胞癌中的EZH2表达高于非癌组织,中低分化鳞癌组织表达高于高分化组织,有淋巴结转移组织表达高于无转移组织,临床TNM分期Ⅱ、Ⅲ期患者表达高于Ⅰ期患者,低于Ⅳ期患者,高表达组患者术后远期生存时间较低表达组明显降低(P＜0.05).结论 EZH2促进食管鳞癌的淋巴结转移,降低分化程度.术后患者EZH2高表达组较低表达组预后差.%Objective To investigate the expression of enhancer of zeste homolog 2 (EZH2) in esophageal squamous cell carcinoma and its association with the prognosis of postoperative patients. Methods Surgical specimens were obtained from 102 patients with esophageal squamous cell carcinoma undergoing radical resection in our hospital from 1996 to 2006. Immunochemistry was employed to examine EZH2 protein expressions in the specimens, including 102 carcinoma tissue specimens, 30 adjacent tissue specimens and 30 normal esophageal tissue specimens. The expression levels of EZH2 were analyzed in relation to the clinicopathological parameters of the patients including gender, age, tumor differentiation, TNM, and lymph node metastasis. The postoperative patients were followed up to analyze the association of EZH2 expression with the clinical outcomes. Results The esophageal squamous cell carcinoma tissue showed a higher EZH2 expression than the adjacent and normal esophageal tissues. EZH2 expression was higher in poorly differentiated carcinoma than in well differentiated tissue, and also higher in cases with lymph node metastasis than those without; the expression was higher in TNM stage Ⅱ/Ⅲ patients than in stage I patients but lower than in stage Ⅳ patients. The patients with low EZH2 expression was found to have a longer survival time than those with high EZH2 expression (P<0.05). Conclusion EZH2 plays an important role in the differentiation and metastasis of esophageal squamous cell carcinoma, and a high EZH2 expression is associated with a poor outcome in the the postoperative patients.