脂肪酶促Ketoprofen对映体选择性酯化反应--有机溶剂、助溶剂及添加剂的影响

摘要

The effect of reaction media, cosolvent and additives on candida antarctica lipase B(Novozym 435)-catalyzed enantioselective esterification of ketoprofen was systematically exp-lored. Novozym 435 showed high catalytic activity and enantioselectivity in cyclohexane. Cosolvent and additives have profound effects on Novozym 435-catalyzed enantioselective esterification of ketoprofen: polar cosolvent benzene and toluene can improve enzymatic enantioselectivity; a small amount of 18-Crown-6 could increase both the esterification rate and the enantioselectivity. Dimethylsulfoxide (DMF) however, could not improve the enanti-oselective esterification within the scope studied.%系统研究了有机介质、助溶剂及添加剂对脂肪酶Novozym 435(Candida antarctica lipase B)催化的Ketoprofen的对映体选择性酯化反应的影响.以环己烷为反应介质,Novozym 435表现出较高的活性和对映体选择性;助溶剂及添加剂对Ketoprofen的立体选择性酯化反应有较大影响: 极性助溶剂苯和甲苯能提高酶反应的对映体选择性;添加少量的18-Crown-6能提高酯化反应速率和对映体选择性;在研究的范围内,N,N-二甲基甲酰胺(DMF)不能提高酶反应对映体选择性.