活的非可培养状态金黄色葡萄球菌的诱导和复苏后菌株的生理特征变化

摘要

将金黄色葡萄球菌Staphylococcus aureus接种在含有一定浓度的山梨酸钾的溶液中,在不同温度下诱导其进入活的非可培养状态(viable but non-culturable,VBNC),采用脑心浸液培养基(BHI)升温的方法复苏VBNC状态的菌株,并考察了复苏菌株的生理特性及对环境的应激抵抗力.结果表明,诱导后金黄色葡萄球菌进入VBNC状态,经BHI升温方法复苏后的菌体和正常状态的菌体形态上并无区别.在血平板上,复苏后的金黄色葡萄球菌菌落呈乳白色,且无溶血圈.生化试验表明,复苏的菌株除尿素酶阴性外,其他代谢特征均与标准菌株相同.复苏后的菌株对环境的应激抵抗力减弱,可能与其产类胡萝卜素的能力有关.%Staphylococcus aureus was induced into viable but non-culturable (VBNC) state by low temperature and potassium sorbate.The resuscitation of VBNC strain was successful when being incubated in brain heart infusion (BHI) medium.The physiological metabolisms and responses to stresses of the resuscitative cells were also studied.The results showed that the morphology and the physiological characteristics of the resuscitative strains were the same as the standard strain except for the negative urease.On blood agar plate,the colonies of the resuscitative strains were white,without hemolysis.Furthermore,the resuscitative strains were more susceptible to stress.This may be related to the lack of ability of carotenoid biosynthesis.