20味中药提取物对无乳链球菌的体外抑菌活性研究

摘要

【目的】筛选对无乳链球菌Streptococcus agalactiae具有良好体外抑菌效果的中药．【方法】通过乙醇回流、超声波方法提取20味中药的抗菌活性部位，采用试管二倍稀释法测定黄连Coptis chinensis等20味中药提取物对无乳链球菌的最低抑菌浓度（ Minimal inhibitory concentration ，MIC）和最低杀菌浓度（ Minimal bactericidal concentration ， MBC），并评价抗菌活性较强的几味中药的体外联合抑菌效果．【结果和结论】乌梅Prunus mume、黄连、黄芩Scutel-laria baicalensis、虎杖Polygonum cuspidatum和地榆Sanguisorba officinalis 5味中药提取物对无乳链球菌的MIC范围为7.80～31.25 mg/mL．蒲公英Taraxacum mongolicum、茵陈Artemisia scoparia、秦皮Fraxinus rhynchophylla、艾叶Arte-misia argyi、黄柏 Phellodendron chinense、鱼腥草 Houttuynia cordata、紫花地丁 Violae yedoensis 和白头翁 Pulsatilla chinensis 8味中药提取物的MIC范围为62.50～125.00 mg/mL．何首乌Po．multiflorum、苦参Sophora flavescens、穿心莲Andrographis paniculata、杜仲Eucommia ulmoides、连翘Forsythia suspensa、金银花Lonicera japonica和夏枯草Prunel-la vulgaris 7味中药提取物的MIC范围为250.00～500.00 mg/mL．联合抑菌试验结果表明，黄芩、虎杖、地榆和黄连的联合抑菌指数（FICI）≤1，虎杖、黄芩和地榆的FICI＞2．黄连、黄芩、虎杖和地榆对无乳链球菌均具有很强的体外抗菌活性，地榆、黄连为相加作用，黄连、黄芩和虎杖为协同作用，地榆与黄芩、虎杖为拮抗作用．%Objective] Screening for the traditional Chinese medicines which have good antibacterial effects on Streptococcus agalactiae in vitro.[Method] Using ethanol reflux and ultrasonic method , ex-tracts of 20 different traditional Chinese medicines were obtained .Minimal inhibitory concentration ( MIC) and minimal bactericidal concentration ( MBC) of Coptis chinensis and other 19 extracts of tradi-tional Chinese medicines on St.agalactiae were detected by macrodilution broth method , and combined antibacterial effects in vitro of them were also observed .[Result and conclusion] The MICs of the ex-tracts obtained from Prunus mume, Coptis chinensis, Scutellaria baicalensis , Polygonum cuspidatum and Sanguisorba officinalis against St.agalactiae ranged from 7.80 to 31.25 mg/mL.The MICs of the ex-tracts obtained from Taraxacum mongolicum, Artemisia scoparia, Fraxinus rhynchophylla, Artemisia arg-yi, Phellodendron chinense, Houttuynia cordata, Violae yedoensis and Pulsatilla chinensis ranged from 62.50 to 125.00 mg/mL.The MICs of the extracts obtained from Po.multiflorum, Sophora flavescens, Andrographis paniculata, Eucommia ulmoides, Forsythia suspensa, Lonicera japonica and Prunella vul-garis ranged from 250.00 to 500.00 mg/mL.The results of joint bacteriostatic test showed that the frac-tional inhibitory concentration index ( FICI) of the extracts obtained from Sc.baicalensis, Po.cuspida-tum, Sa.officinalis and C.chinensis was less than or equal to 1.The FICI of Po.cuspidatum, Sc.ba-icalensis and Sa.officinalis was more than 2.C.chinensis, Sc.baicalensis, Po.cuspidatum and Sa.offi-cinalis showed a good antibacterial effect against St.agalactiae in vitro.The combination of Sa.officina-lis and C.chinensis was characterized by a additive effect , whereas the combination of C.chinensis, Sc. baicalensis and Po.cuspidatum was characterized by a synergism effect .The combination of Sa.officina-lis and Sc.baicalensis, Po.cuspidatum showed antagonism effect .