The effects of different pretreatments on enzymatic hydrolysis and ethanol fermentation in maize straw were investigated. The maize straw was pretreated by grinding, steam explosion (SE) and liquid hot water (LHW), respectively. The glucose concentration, hydrolysis rate, ethanol yield and inhibitor concentrations (acetic acid, furfurol and methylfurfurol) in enzymatic hydrolysate through different pretreatments were determined. The results showed that LHW pretreatment could significantly improve the hydrolysis rate, under the conditions of solid-liquid ratio 2:10 and time 48 h, the glucose concentration is 60.6 g·L-1 and hydrolysis rate was 63.13%. In addition, the inhibitor concentrations were very low. 28.29g·L-1 ethanol was obtained and ethanol productivity was up to 46.68%. The theoretic rate for ethanol is 91.5%. Superfine grinding of LHW method effectively improved enzymolysis rate and ethanol productivity.%研究不同预处理方法对玉米秸秆酶解和乙醇发酵的影响.比较玉米秸秆经粉碎、汽爆和水热3种预处理后酶解液葡萄糖含量、酶解率、乙醇得率以及发酵液中抑制物乙酸、糠醛和羟甲基糠醛的含量,对不同预处理方法进行评价.结果表明:水热超细玉米秸秆能有效提高酶解率,在固液比2:10,酶解48h时,生成葡萄糖含量为60.6g· L-1,纤维素酶解率为63.13%,并且产生的乙酸、糠醛和羟甲基糠醛的含量很低;以此水解液发酵生产乙醇,乙醇含量为28.29g·L-1,乙醇得率为46.68%,为理论乙醇得率的91.5%.说明采用水热超细秸秆可有效提高纤维素酶解率和乙醇得率.
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