为研究蜂蜜对三聚氰胺及其同系物作用对小鼠免疫细胞的影响,同时研究蜂蜜作为天然解毒剂的作用,本试验以KM小鼠作为试验动物,分为三聚氰胺组、三聚氰胺+三聚氰酸联合组、三聚氰胺+蜂蜜组、联合+蜂蜜组及对照组5个试验组.小鼠隔天灌胃28d后,经眼球采血,采用全自动血液分析仪和流式细胞仪检测外周血和淋巴细胞亚群的变化.结果表明:三聚氰胺与三聚氰酸联合攻毒能导致白细胞和淋巴细胞数目显著升高(p<0.05);与空白对照组相比,联合攻毒组小鼠CD4+/CD8+值显著下降(p<0.05);从CD138+表达率上看,单独及联合攻毒组均显著降低(p<0.05).三聚氰胺类物质能够影响机体免疫细胞的应答反应,对小鼠免疫功能产生一定的抑制作用,而蜂蜜对这种抑制具有一定的缓解作用.%The effect of honey on the immune cells of mice affected by melamine and its homologues was investigated, and the detoxification effect of honey was also studied. The KM mice were divided intofive groups: melamine group, melamine + cyanuric acid combined group, melamine + honey group, combined + honey group and control group. After 28 days' by intragastric administration, blood samples were taken from the eyeballs. The peripheral blood and lymphocyte subsets were detected by automatic hematology analyzer and flow cytometer. The results showed that the combination of melamine and cyanuric acid could significantly increase the number of leukocytes and lymphocytes (p<0.05). Compared with the control group, the ratio of combined group mice CD4+/CD8+ was significantly decreased (p<0.05). While the expression rate of CD138+, alone and combined groups were significantly decreased (p<0.05). Melamine had some effect on the responses of immune cells of immune cells, partially inhibited the immune function of mice; Honey had mitigation effects on this kind of inhibition.
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