A novel superoxide dismutase gene was cloned from mussel Hyriopsis cumingii ( named HcSODn ) by reverse transcriptional polymerase chain reaction ( RT-PCR) and rapid amplification of cDNA ends ( RACE) techniques. The results show that the full length cDNA of HcSODn gene is of 1575 bp, containing an 804 bp open reading frame encoding a protein of 267 amino acids and covering a highly conserved Cu_Zn_SOD domain. HcSODn mRNA is ubiquitously expressed in all tissues examined, but mainly detected in the gill. The relative expression level of HcSODn gene is up-regulated significantly in hemocytes at 3 h when it is challenged by Aero-monas hydrophila (P<0. 05), indicating the important roles of HcSODn in innate immune responses of mollusks.%采用RT-PCR和RACE技术,从三角帆蚌( Hyriopsis cumingii)中克隆到了一个新型超氧化物歧化酶基因(命名为HcSODn). HcSODn基因的长度为1575 bp,包括一个长804 bp的开放阅读框,编码267个氨基酸的蛋白,并含有一个保守的Cu_Zn_SOD结构域. HcSODn mRNA在所检测的三角帆蚌的各个组织中均有表达,而腮中最高.在嗜水气单胞菌诱导下,血细胞中的HcSODn mRNA的表达水平在3 h时显著上调(P<0.05),表明HcSODn在三角帆蚌抗细菌感染的天然免疫反应过程中发挥重要作用.
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