Objective To investigate the effects of fastigial nucleus electrical stimulation ( FNS ) on the serotonin ( 5-hydroxytryptamine,5-HT) contents in brain tissues of rats after myocardial infarction( MI), and to explore the role of 5-HT in the prevention of sudden cardiac death ( SCD ) after FNS. Methods Ninety SD rats were randomized into 3 groups with 30 rats in each group: MI group, FNS group and fastigial nucleus lesion group. In MI group, left anterior descending artery of rats was ligated. In FNS group,rats were pretreated with fastigial nucleus electro-stimulation for 1 h,and then left anterior descending artery was ligated. In fastigial nucleus lesion group,rats were treated with fastigial nucleus electro-stimulation for 1 h after fastigial nucleus was damaged for 5 d, and then left anterior descending artery was ligated. Each group was subdivided into 3 subgroups according to the three time points of 1, 7,21 d after ligation of left anterior descending artery. Eight SD rats were chosen as the sham operation group. At days 1,7,21 after ligation of left anterior descending artery, the contents of 5-HT were determined in brain tissues with fluorospectrophotometry. Results (T)The levels of 5-HT in MI group and fastigial nucleus lesion group were significantly lower than that in sham operation group at days 1,7 after myocardial infarction ( P < 0.05 ), but 5-HT in FNS group was significantly higher than in MI group( P < 0. 05 ). There was no significant difference in 5-HT between MI group and fastigial nucleus lesion group( P > 0.05 ). (2) At day 21 after myocardial infarction, there was no significant difference in 5-HT levels among 4 groups. Conclusion FNS can increase the levels of 5-HT in brain tissues at an early stage after myocardial infarction, which may be one of mechanism in the prevention of SCD.%目的 通过研究小脑顶核电刺激(FNS)对心肌梗死(MI)大鼠脑组织神经递质5-羟色胺(5-HT)的影响,探讨5-HT在FNS干预心脏性猝死(SCD)中的作用.方法 SD大鼠90只,随机分为3组,每组30只:MI组,仅结扎左冠状动脉前降支(LAD);FNS组,预先FNS 1 h再予以LAD结扎;小脑顶核毁损组,毁损小脑顶核5 d后FNS 1 h,再行LAD结扎;各组又分MI后1,7,21 d 三个亚组.另取8只设为假手术组.LAD结扎1,7,21 d后,应用荧光分光光度法测定大鼠脑组织内5-羟色胺含量.结果 ①LAD结扎1,7 d 后,与假手术组比较,MI组和小脑顶核毁损组大鼠脑组织5-HT均明显降低(P<0.05);与MI组比较,FNS组大鼠脑组织5-HT明显增加(P<0.05);小脑顶核毁损组与MI组脑组织5-HT含量比较差异无统计学意义(P>0.05).②LAD结扎21 d后,各实验组间大鼠脑组织5-HT含量比较无统计学差异.结论 FNS可增加心肌梗死大鼠早期脑组织5-HT含量,并可能通过增加5-HT含量来控制心脏性猝死的发生.
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