目的 建立荧光金逆行标记乳鼠视网膜神经节细胞的方法.方法选取出生1 d的SD大鼠乳鼠,腹腔注射水合氯醛麻醉后,剪开头部皮肤,向双侧上丘各注射4%荧光金注射液1 μl,缝合皮肤,4 d后处死,提取视网膜,胰酶消化后培养,显微镜下观察被标记的视网膜神经节细胞.结果 混合培养的视网膜细胞中,可见被荧光金标记的视网膜神经节细胞,大约占细胞总数的0.34%,视网膜神经节细胞可存活3周.结论 荧光金逆行标记乳鼠视网膜神经节细胞是一种可行的方法,可用于视网膜神经节细胞的鉴定.%Objective To establish a method to label the retinal ganglion cells of neonatal rats with fluorogold. Methods One-day neonatal Sprague-Dawley rats were anesthetized by intraperitoneal injection with chloral hydrate, and then 1 μ1 of 4% fluorogold was mi-croinjected bilaterally into the superior colliculi of rats. Four days later, primary mixed retinal cell cultures were established from retinas of five-day neonatal rats. Results Under fluorescence microscope, retinal ganglion cells in mixed cultured retinal cells were successfully labeled with fluorogold, which accounted for 0. 34% of total retinal cells. In addition, retinal ganglion cells survived for three weeks in vitro. Conclusion Retinal ganglion cells of neonatal rats labeled by fluorogold is a feasible approach, and could contribute to the I-dentification of retinal ganglion cells in primary mixed retinal cell cultures.
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