Objective To establish the mouse neuroblastoma xenograft model in BALB/c mice, and to explore the influential factors for the successful rate of modeling. Methods BALB/c mice were randomly divided into three groups: abdominal cavity group, nape group and oxter group. Neuro-2a( N2a )cells were collected and condensed as 1 × 10 /ml during the logarithmic growth phase. BALB/c mice were injected subcutaneously 0. 2 ml N2a cells through abdominal cavity, nape and oxter,respectively. The mice were injected for the first cycle at days 1-7 after born. Based on the examination of appearance and touch,the mice without neuroblastoma were injected a-gain for the second cycle( 8-14 d ), and even for the third cycles( 15-21 d )if necessary in order to have successful inoculation. The latent period and rate of tumor bearing were recorded, and the growth curves of nomal mice and the ones bearing cancer. Results The survival rate of mice was 94. 34%( 50/53 )and the tumour-bearing rate was 42% . Of all the tumor-bearing mice,tumour formation rate was 46. 2% via abdominal cavity, 38. 1% via nape and 33. 3% via oxter. The tumor-bearing rate of female mice was higher than that of male ones( 57. 89% vs 32. 26% ,P ＜ 0. 05 ). The tumor generation rate decreased with the aging, and the tumour formation rate was 48. 2% for 1 -7 d old mice,35. 7% for 8 - 14 d old mice and 20% for 15 -21 d old mice. The shortest latency was 5 d( 4. 76% ),and the longest was 20 d( 9. 53% ), while the most was 11 - 15 d( 61. 9% ). Finally the mice bearing cancer died of excessively bigger neu-roblastoma and skeletization. Conclusion Mouse neuroblastoma xenograft model is successfully established in the immunocompetent BALB/c mice,which paves the way for the further research on pathogenesis and therapy of neuroblastoma.%目的 通过在BALB/c小鼠不同部位注射神经母细胞瘤(N2a)细胞建立转移瘤模型,探讨BALB/c小鼠影响造模成功的因素.方法 BALB/c小鼠以注射部位随机分为腹腔、颈背、腋下3个实验组.注射液为用完全培养液和处于对数生长期的N2a细胞配制成含1×107个/ml的细胞悬液,0.2 ml/只皮下注射.实验鼠首次注射在出生1-7 d进行,根据外观和触摸检查对确定未长瘤的鼠只在8-14 d进行再注射;并对仍确定未长瘤者在15-21 d时进行了第3次注射.记录BALB/c小鼠成瘤的潜伏期、成瘤率,及绘制正常鼠与荷瘤鼠的生长曲线等.结果 在53只参与实验的BALB/c小鼠中,有50只完成了实验,存活率为94.34%;其中21只成功建模,成瘤率为42%.就接种部位来看,腹腔、颈背和腋下的成功率分别为46.2%,38.1%,33.3%;雌性的成瘤率为57.89%,高于雄性的32.26%(P<0.05);成瘤率随着接种年龄的增长而降低,3个年龄段的成瘤率分别为48.2%,35.7%,20%;荷瘤鼠的潜伏期最短的5 d,最长的在20 d以上,大多在11-15 d,三者分别占荷瘤鼠的比例为4.76%,9.53%和61.9%.荷瘤鼠最终因瘤体过大、极度消瘦而死亡.结论 本实验利用完全免疫的BALB/c小鼠成功建立了神经母细胞瘤移植模型,为进一步研究神经母细胞瘤的发病机制和治疗提供了理想的实验材料.
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