kshv-mir-k12-1-5p相关基因在卡波西肉瘤中的表达

摘要

目的 探索kshv-mir-k12-1-5p相关基因在卡波西肉瘤(KS)组织中的表达意义. 方法 通过在线miRNA靶基因软件Mirbase、Miranda和Targetscan寻找kshv-mir-k12-1-5p的靶基因.通过GO和KEGG分析靶基因的信号通路及通路上的关键基因.使用免疫组化的方法检测靶基因、关键基因(kshv-mir-k1 2-1-5p相关基因)在卡波西肉瘤组织的表达. 结果 kshvmir-k12-1-5p的靶基因为CDKN1A,CDKN1A信号通路为Cell cycle通路,通路下游关键基因为CyclinD1、CyclinE.CDKN1A、CyclinD1、Cyclin E在肿瘤组织与瘤旁组织中的表达差异有统计学意义(P＜0.05),CDKN1A在卡波西肉瘤中的阳性表达率低于瘤旁组织(P＜0.05),CyclinD1、CyclinE在卡波西肉瘤中的阳性表达率高于瘤旁组织(P＜0.05);在KS中CyclinD1与年龄相关(P＜0.05),CyclinE与民族相关(P＜0.05);在KS中CDKN1A与CyclinD1、CyclinE均呈负相关(P＜0.05).CyclinD1与CyclinE呈正相关(P＜0.05). 结论 kshv-mir-k12-1-5p的相关基因:CDKN1A、CyclinD1、CyclinE表达异常在KS发生、发展中具有重要的作用.%Objective To explore the expression of kshv-mir-k12-1-5p-related gene in Kaposi's sarcoma (KS) and its significance.Methods The kshv-mir-k12-1-5p target gene was detected through Mirbase,Miranda and Targetscan of the online miRNA target gene software.GO and KEGG were used to analyze signaling pathway and key gene in the pathway of the target gene.The expression of target gene and key gene(kshv-mir-k12-1-5p-related gene) was detected in Kaposi's sarcoma by immunohistochemistry.Results The target gene of kshv-mir-k12-1-5p was CDKN1A,CDKN1A signaling pathway was the cell cycle pathway,and the downstream key genes were CyclinD1 and CyclinE.Positive expression rate of CDKN1A in KS was lower in tumor tissues than that of adjacent tissues(P ＜ 0.05).Positive expression rates of CyclinD1,CyclinE in KS were higher in tumor tissues than that of adjacent tissues (P ＜ 0.05).Expression of CyclinD1 in KS was associated with age (P ＜ 0.05).Expression of CyclinE in KS was associated with nation (P ＜ 0.05).There was a negative correlation between the expression of CDKN1A and CyclinD1,CyclinE in KS (P ＜ 0.05).There was a positive correlation between the expression of CyclinD1 and CyclinE in KS(P ＜ 0.05).Conclusion The expression of kshv-mir-k12-1-5p-related genes CDKN1A,CyclinD1,and CyclinE may play an important role in the occurrence and development of KS.