IL-35在肺炎支原体感染患者血清的表达水平及在不同性别、年龄的差异

摘要

目的 分析不同年龄段肺炎支原体感染患者血清中白细胞介素35(IL-35)的水平,探讨其与性别、年龄的相关性以及在肺炎支原体感染过程中的作用. 方法 将纳入标准的94例肺炎支原体感染阳性作为研究组,69例肺炎支原体阴性作为对照组.符合入选标准的研究组与对照组按年龄分为三组:分别是≤1岁组(n=56),＞1-10岁组(n=54)和＞60岁组(n=53).通过ELISA方法分别检测各年龄组中血清IL-35水平,分析研究组与对照组血清IL-35水平在不同年龄组之间的表达差异以及相关性. 结果 ≤1岁组中研究组IL-35水平[(41.65±28.80) ng/L]高于对照组[(27.87±19.69) ng/L,P=0.041],＞1-10岁组中研究组IL-35水平[(18.55±lO.08) ng/L]低于对照组[(30.46±10.61) ng/L,P=0.000],＞60岁组中研究组IL-35水平[(33.22±14.65) ng/L]高于对照组[(22.61-±8.92) ng/L,P=0.002];对照组血清IL-35水平在不同年龄组两两比较差异无统计学意义(P =0.226);研究组血清IL-35水平≤1岁组高于＞1-10岁组(P=0.000);＞60岁组高于＞1-10岁组(P =0.002);≤1岁组与＞60岁组之间差异无统计学意义(P =0.086 ＞0.05). 结论 IL-35可能在肺炎支原体感染过程发挥了抗炎症作用.对照组与研究组血清中均存在IL-35表达,其表达水平与性别、年龄没有相关性,血清IL-35水平可以考虑作为肺炎支原体感染控制水平评估的免疫学参考指标.%Objective To investigate the concentration of IL-35 in serum of patients infected with Mycoplasma pneumoniae(MP) and its difference in different age and gender,and to explore its possible roles in the process of infection.Methods Ninety-four patients infected with MP were selected as experimental group and 69 controls without infection were included as control group.All subjects were divided into three groups:less than 1 year old group,＞ 1-10 year-old group and over 60-year-old group.The serum IL-35 concentration was detected in experimental groups and control group,and then the difference of serum IL-35 concentration among different age and gender patients was analyzed.Results Serunm IL-35 concentration(ng/ml) in less than 1-year-old patients was higher in experimental group than in control group (41.65 ± 28.80 vs 27.87 ± 19.69,t =-2.090,P =0.041).Serum IL-35 concentration (ng/ml) in ＞ 1-10-year-old patients was lower in experimental group than in control group(18.55 ± 10.08 vs 30.46 ± 10.61,t =4.4145,P ＜ 0.001).Serum IL-35 concentration(ng/ml) was higher in over 60-year-old patients in experimental group than in control group (33.22 ± 14.65 vs 22.61 ± 8.92,t =-3.227,P =0.002).There was no significant difference in serum IL-35 concentrations among controls with different age.The serum IL-35 level in less than 1-year-old group and over 60-year-old group was higher than that in ＞1-10 year-old group.Conclusion IL-35 may play an anti-inflammatory in Mycoplasma pneumoniae infection.The IL-35 expression in serum shows significant difference between MP infection patients and controls.Serum IL-35 concentration may be used as an immunological indicator to test MP infection.